This document is a product data sheet for a rabbit monoclonal antibody (clone EP1536Y) that specifically detects alpha-synuclein phosphorylated on serine 129. It includes reactivity data for human, mouse, rat, and other species across multiple applications, storage and handling instructions, target information, and product promise details.
This document is a product data sheet for Anti-Alpha-synuclein (phospho S129) antibody [EP1536Y] (ab51253), a rabbit monoclonal antibody from Abcam. It is intended for researchers studying Parkinson's disease and other synucleinopathies, and is validated for use in Western Blot, IHC-P, IHC-FrFl, ELISA, and Dot Blot.
The antibody is an IgG isotype, hosted in rabbit, and supplied as a liquid in PBS with 40% glycerol and 0.05% BSA. It is purified by affinity chromatography using Protein A. The immunogen is proprietary. The antibody specifically detects alpha-synuclein only when phosphorylated on Ser129.
Reactivity data is provided for tested species (human, mouse, rat) and expected or predicted species (cow, pig, chimpanzee, macaque monkey, gorilla, orangutan, spider monkey) across applications. Dilution recommendations and notes are given for each application, such as heat-mediated antigen retrieval for IHC-P and PFA treatment for WB.
Target information describes the SNCA gene product, its function in synaptic vesicle trafficking and neurotransmitter release, and its role in dopamine neurotransmission. The document also covers storage conditions (short-term at 4 degrees C, long-term at -20 degrees C) and advises against freeze-thaw cycles.
The product promise section defines tested, expected, predicted, and not recommended combinations for species and applications. The document includes product images for Western blot and immunohistochemistry, showing staining in rat brain lysates and human Parkinson substantia nigra tissue.